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Cisplatin-resistant TGCT cells have a less robust transcriptional response to cisplatin compared to parental cells. ( A ) Volcano plots of the cisplatin response of 2102EP parental and three independently derived cisplatin-resistant cell derivatives. ( B ) Volcano plots of the cisplatin response of NT2/D1 parental and two independently derived cisplatin-resistant cell derivatives. ( C ) Volcano plots of the cisplatin response of 833K parental and two independently derived cisplatin-resistant cell derivatives. ( D ) Number of genes upregulated and downregulated (>1.3 fold change and an FDR < 0.01) after cisplatin treatment in indicated parental and resistant cells.

Journal: Cancers

Article Title: Comparative Analysis of the Transcriptomic Response to Cisplatin in Drug-Sensitive and Drug-Resistant Testicular Germ Cell Tumors

doi: 10.3390/cancers18040575

Figure Lengend Snippet: Cisplatin-resistant TGCT cells have a less robust transcriptional response to cisplatin compared to parental cells. ( A ) Volcano plots of the cisplatin response of 2102EP parental and three independently derived cisplatin-resistant cell derivatives. ( B ) Volcano plots of the cisplatin response of NT2/D1 parental and two independently derived cisplatin-resistant cell derivatives. ( C ) Volcano plots of the cisplatin response of 833K parental and two independently derived cisplatin-resistant cell derivatives. ( D ) Number of genes upregulated and downregulated (>1.3 fold change and an FDR < 0.01) after cisplatin treatment in indicated parental and resistant cells.

Article Snippet: The human TGCT embryonal carcinoma (EC) cell lines NT2/D1, 833K, and 2102EP were obtained from ATCC.

Techniques: Derivative Assay

p53/TFRC1/MYC target genes are associated with disease-free survival in TGCT patients. High expression of a partially overlapping subset of genes associated with p53 activation and TFRC1 and MYC repression were associated with increased disease-free survival in TGCT patients as assessed by Kaplan–Meier log-rank tests. HR, hazard ratio; CI, confidence interval.

Journal: Cancers

Article Title: Comparative Analysis of the Transcriptomic Response to Cisplatin in Drug-Sensitive and Drug-Resistant Testicular Germ Cell Tumors

doi: 10.3390/cancers18040575

Figure Lengend Snippet: p53/TFRC1/MYC target genes are associated with disease-free survival in TGCT patients. High expression of a partially overlapping subset of genes associated with p53 activation and TFRC1 and MYC repression were associated with increased disease-free survival in TGCT patients as assessed by Kaplan–Meier log-rank tests. HR, hazard ratio; CI, confidence interval.

Article Snippet: The human TGCT embryonal carcinoma (EC) cell lines NT2/D1, 833K, and 2102EP were obtained from ATCC.

Techniques: Expressing, Activation Assay